Silencing TCAB1 suppresses proliferation of hepatocellular carcinoma cells by inducing apoptosis

Abstract Objectives Telomerase Cajal body protein 1 (TCAB1) is closely related to the occurrence, development and prognosis of tumors, may affect sensitivity tumor radiotherapy. There are no reports about effect TCAB1 gene expression on proliferation apoptosis HEPG2 cells. We plan investigate whether silencing using siRNA helpful for diagnosis treatment hepatocellular carcinoma. Methods Three sequences (siTCAB1-1, siTCAB1-2, siTCAB1-3) targeting one negative sequence (NC) were designed, synthesized then transfected into cells, separately. The expressions telomerase reverse transcriptase (TERT) in mRNA level detected by RT-qPCR Western blot assays. Moreover, cells measured MTT assay, flow cytometry, respectively. Results data both showed that siTCAB1 group was significantly lower than blank control NC groups (p < 0.05). However, TERT not different among those > result apoptotic rate increased compared with 0.01). Conclusions Silencing can inhibit promote providing a potential therapeutic method